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Common steps for making a Frozen OCT TMA block with our standard Quickarrayer kit

Step 1:
Pre-chill the Quickarrayer kit in freezer

Frozen_Step1.jpg

Step 2:
Turn on the LED light on the Quickarrayer. Assemble a blank frozen OCT recipient block in the arrayer in a low temperature environment, such as a cryostat chamber.

Frozen_Step2.jpg

Step 3:
Punching a hole in the recipient block through the guide plate in a cold chamber.

Frozen_Step3.jpg

Step 3a:
Recipient block with an empty hole.

Frozen_Step3a.jpg

Step 4:
Obtain a sample core from a frozen OCT embedded donor tissue block with an appropriate coring pen in a cold chamber.

Frozen_Step4.jpg

Step 5:
Eject the sample core inside the coring pen collected in Step 4 into the previously made hole (Step 3a) through the guide plate in a cold chamber.

Frozen_Step5.jpg

Step 5a:
Completion of a frozen OCT TMA block. By repeating Step 1 to 5, TMA blocks of frozen samples with different core sizes and layouts can be easily and precisely created.

Frozen_Step6.jpg
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